The cell growth curve is a common method for determining the absolute growth of cells, and is an important indicator for determining cell viability. After the cells are passaged, they are briefly suspended and then attached to the wall, and then the different incubation periods are passed, that is, into the exponential growth phase of massive division. In order to accurately describe the dynamic changes of the number of cells in the whole process, the typical growth curve can be divided into a slow growth latency, a large gradient exponential growth phase, a platform-like flat top period and four stages of degradation and decline.
The anesthetic mask can be attached to the patient's face, delivering oxygen and volatile anesthetic gas to the patient.
Oxygen Masks are devices that are constructed to supply oxygen or other gases to an individual. Masks of this type fit snugly over the nose and mouth, and are equipped with a tube that connects the Oxygen Mask to a storage tank where the oxygen is contained. The Oxygen Mask is made from PVC, as they are light in weight, they are more comfortable than some other masks, increasing patient acceptance.
The Nebulizers are connected by tubing to a compressor, that causes compressed air or oxygen to blast at high velocity through a liquid medicine to turn it into an aerosol, which is then inhaled by the patient, and the medicine in the form of a liquid solution is loaded into the device upon use. The Nebulizers are commonly used for patients in hospitals who have difficulty using inhalers, such as in serious cases of respiratory disease, or severe asthma attacks, it is also for ease of use with young children or the elderly.
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Principle of experimental method Cell growth curve is an important method to observe the basic law of cell growth. Only cells with their own stable growth characteristics are suitable for use in experiments to observe changes in cell growth. Therefore, in the observation of the growth characteristics of cell line cells and non-lineage cells, the measurement of the growth curve is the most basic indicator. The measurement of the cell growth curve can generally be carried out using a cell counting method. Experimental Materials Reagents, kits Instruments, consumables Experimental procedure
2. Count and accurately inoculate the cells in 21 to 30 well-sized flasks or culture wells (with 24-well culture plates). The total number of cells per well or well should be the same, and the amount of the culture solution should be the same.
3. Three bottles (well) of cells were taken every day or every other day for counting and the mean was calculated. For 3 to 5 days of culture, it is often necessary to change the cells of the uncounted cells.
4. The culture time is the horizontal axis, and the number of cells is the vertical axis (logarithm). It is drawn on the semi-logarithmic coordinate paper and connected to the curve to form the growth curve of the cell. Precautions
2. Many laboratories now use the MTT method to determine the growth curve using a culture plate, which is relatively simple.
3. The standard cell growth curve is similar to the "S" shape. Generally, the number of cells decreases on the first day after passage, and after a few days of late adaptation, then enters the logarithmic growth phase, and the growth is stable after the plateau. Arrived at aging.
4. The time when the number of cells increases by a factor of two on the growth curve is called the cell doubling time and can be converted from the curve.
Cell Technology Topics: Cell Growth Curve Experiment
cell
D-Hanks liquid bovine serum RPMI1640 double antibody 0.08% trypsin
Purification Workbench Centrifuge Constant Temperature Water Bath Refrigerator Inverted Phase Difference Microscope Incubator Straw Culture Bottle Tip Head 24 Culture Plate Rubber Plug Micro-Sample Gun Red Blood Cell Counting Plate
1. The cells in a good growth state were digested by a general passage method to prepare a cell suspension.
1. Although the cell growth curve is the most commonly used, sometimes its reflection value is not accurate enough, and it can have an error of 20 to 30%. It needs to be combined with other indicators for analysis.