1. What experiments are suitable for D-fluorescein? D-fluorescein potassium salt and 5-fluoro-luciferin, as substrates for Luciferase, are commonly used in the following experiments: 2. The basic principle of D-fluorescein applied to living imaging The chemiluminescence reaction in which D-luciferase is involved in the cell follows the basic principle of the enzymatic reaction. In the case of excess substrate D-fluorescein potassium, the photon number (luminescence amount) produced by the reaction and D-luciferin in the cell The amount of enzyme is positively correlated so that the number of cells can be derived. 3. Stability of D-fluorescein potassium salt solution D-fluorescein potassium salt is easily soluble in water and buffer, and its solubility can be as high as 25 mg/mL. The concentration is generally 3-15 mg/mL. The pH of the solution, the oxygen in the solution, and the storage time are important for its stability during storage. In the case where the pH of the solution is < 6.5 (hydrolysis) or > 7.5 (the racemization occurs, the D form is converted to the L form), the D-luciferin potassium salt is relatively unstable. If a small amount of oxygen is present in the solution, the rate of degradation of D-luciferin potassium will be accelerated. After D-fluorescein is formulated into a solution, under the optimal storage conditions (-80 ° C, optimal pH, no oxygen), there is a fixed degradation rate: 0.2% / day, so D-fluorescence stored in solution It should be used within 1 year. In the presence of water, racemization occurs mainly, that is, D-luciferin is converted to L-luciferin, and it has been reported that L-luciferin has an inhibitory effect on luciferase-catalyzed enzymatic reaction. 4. The difference between D-luciferin, sodium D-luciferin and potassium D-fluorescein The main difference is in dissolution and dispersion properties. D-fluorescein potassium salt has high solubility in water and buffer, and the dissolution rate is fast, so D-fluorescein potassium salt is commonly used in general biological tests. 5. Preservation and transportation conditions of D-fluorescein potassium salt Storage conditions: ≤ -20 ° C, protected from light, kept dry. (Can be kept for two years without opening the package). Transportation conditions: High-purity D-fluorescein potassium salt can be stored under normal temperature conditions for a short period of time, and the transportation conditions are normal temperature. The original (not open packaging) fluorescein bottle is filled with argon to ensure its stability. After opening the package, please keep it in strict accordance with the storage conditions. D-fluorescein potassium salt has the property of being easily soluble in water, and it is exposed to the air and is easy to absorb moisture. In the weighing process, please consider the characteristics of its easy moisture absorption, first temperature balance, and then quickly weigh. For product details, please visit: http://?kw2=Luciferin&tn=5 Face Masks,Medical Facemask,Filtration Isolation Medical Facemasks,Anto-Bacteria Face Masks Zhende Medical Co.,Ltd , https://www.zdmedicalproduct.com
1. Reporter gene analysis 2. In vivo imaging
3. In vitro imaging
4. ATP determination 5. Other luciferase and its gene related analysis and research work