product description Fundamental This kit uses a double antibody sandwich method. The anti-Human ApoA1 antibody was coated on the plate, and the ApoA1 in the specimen or standard was bound to the coated antibody during the experiment, and the free component was washed away. Biotinylated anti-Human ApoA1 antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human ApoA1 antibody binds to Human ApoA1 bound to the coated antibody, and biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay analyzer. The ApoA1 concentration and the chemiluminescence value were positively correlated. The standard curve was used to determine the concentration of ApoA1 in the specimen. Steps 1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes. 2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes. 3. Wash 3 times 4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes. 5. Wash 5 times 6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C 7. Readings 8. Calculation of results Sodium Erythorbate Fcc Grade,Sodium Erythorbate Powder,Sodium Erythorbate Food Additive,Food Grade Sodium Erythorbate SHANDONG BAISHENG BIOTECHNOLOGY CO., LTD , https://www.baishengbioproducts.com English name  Human ApoA1 (Apolipoprotein A1) CLIA Kit  Chinese name   Human apolipoprotein A1 (ApoA1) chemiluminescence immunoassay kit   Item number  E-CL-H0121c  species  Human / person  specification  96T/Kit (8*12 strips) 48T/Kit (8*6 strips)  Detection method   Double antibody sandwich method   examination range  3.125~200ng/mL  Sensitivity  1.875ng/mL