At present, the detection of microparticles is mainly detected by ELISA and flow cytometry. The ELISA method is susceptible to interference by other soluble antigens, and the storage time and mode of whole blood or plasma, the processing method of the sample, and different detection methods all affect the results of particle detection. The flow cytometry method provides a more efficient and rapid analysis of particle analysis capabilities. A variety of flow cell markers are available for the detection of microparticles, which are primarily used to detect the activation state of the microparticles and their cellular origin. However, particles detected by flow cytometry are difficult to detect due to wavelength limitation, and particles having a diameter of less than 0.3 μm to 0.5 μm . Apogee flow cytometer Sensitivity 100nm, resolution 10nm : With the high sensitivity and resolution highlighted by Apogee A50 , Apogee A50 can clearly cluster, count and analyze particle samples with a diameter difference of 10nm or more. Fast data acquisition performance, up to 100,000 events/sec , high-concentration particle sample analysis capabilities. Highly sensitive, multi-channel fluorescence detection capability: Flexible use of cMP- specific antigen fluorescent antibodies, accurate analysis of particle function and source. “Overcoming Limitations of Microparticle Measurement by Flow Cytometry†- Romaric, Philippe, SEMINARS IN THROMBOSIS AND HEMOSTASIS/VOLUME 36, NUMBER 8 2010 Particle detection is suitable for a variety of disease studies as follows: Cardiovascular diseases Thrombotic disease Acute coronary syndrome Heparin-induced thrombocytopenia (Heparin-induced thrombocytipenia Atherosclerosis Sickle cell disease Venous thrombosis Paroxysmal nocturnal hemoglobinuria Stroke Stroke ... Autoimmune disease Cardiovascular risk factors Antiphospholipid syndrom Diabetes Diabetes Lupus Lupus Hypertension Hypertension TTP Metabolic syndrome ITP Chronic renal insufficiency PAR Dyslipidemia Dyslipidemia ... Infection, inflammatory disease Tumor Sepsis Sepsis ... HIV
Laser distance measure is a very convenient
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Specification:
Product
Model:
JX40
Accuracy:
±1.5mm (0.06
inch)
Measuring
Unit:
metre/inch/feet
Measuring
Range:
0.03~40m
(0.01 to 131 ft)
Measuring
Time:
0.1~4
seconds
Laser Class:
Class II
Laser Type:
635nm,
<1mW
Waterproof
& Dustproof :
IP54
Auto Laser
Off:
60 seconds
Automatic
Power-off:
480
seconds(8 minutes)
Lighting:
white LED
Datum
Option:
Can choose
Front edge or end piece edge as datum
Battery
Status:
Y
Signal
Strength Display:
Y
LCD display:
2 inch large
screen
Display
illumination and multi-line display:
Max
2 displays
Data Memory:
Y (allow 20
values)
Backlight
display:
Y
Continuous
Measurement:
Y
Min/max
Measurement:
Y
Addition/Subtraction(Distance/Area/Volume):
Y
Area/Volume/Pythagoras
Measurement:
Y
Battery
Type:
AAA alkaline
battery(2*1.5v )
Weight:
about
80g
Dimensions(L*W*H):
4*2*11.5cm
Auto
correction and error report technology:
Y
Operating
Temperature:
0-40 ℃(32-104 ℉ )
Storage
Temperature:
-20~60 ℃
(-4~140 ℉)
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Myeloproferative syndromes )
Flow analysis method in particle research
Circulated microparticles ( cMPs ) are small vesicles with a diameter of less than 1 μm that are detached from the cell membrane after cell activation, injury or apoptosis. In diabetes, cardiovascular disease, AIDS, chronic inflammatory diseases, circulating microparticles are found to be elevated, and some of its biological activities, such as the transmission of intercellular biological activity signals, immune regulation of angiogenic tissue repair, etc., and disease occurrence Development is closely related. The understanding of it is still being explored continuously, and its research is also increasing.
Click on the image below to view a video lecture on circulating particle flow analysis methods.
Circulating microparticles are different from apoptotic bodies and exosomes. Apoptotic bodies are formed by budding when cells are apoptotic.  1 μm ; exosomes are vesicle particles released by cell activation, released in an exocrine form, with a diameter ranging from 10.0 nm to 100 nm.  . The diameter of the circulating particles is approximately  0.1 μm - 1 μm , they have similar basic characteristics to the mother cells.
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