Liver cancer refers to malignant tumors that occur in the liver. The number of new cancer cases in China ranks first in the world, and the number of new cases and deaths of liver cancer ranks first in the world. At present, the incidence of liver cancer in China is about 25.7/100,000, which is the third most serious malignant tumor after gastric cancer and lung cancer. Exploring the biological characteristics, pathogenesis and metastasis mechanism of liver cancer has become a hot topic in current research.

Research on liver cancer is indispensable for cell and animal models. How to choose these tools can help us to conduct better research, obtain positive data, publish papers, apply for funds, and climb the peak of life. Do you know?

1. Which cell model do I need? Is it easy to operate?

Because the cells are easy to operate, the time period is short, and the results are easy to produce, it is definitely the first step in all studies. The following summarizes the characteristics of some commonly used liver cancer cells:

*More comprehensive pre-experiment information, please consult the local salesman

Second, the cell experiment works well, how does the animal experiment do?

After completing the in vitro cell experiments, in order to send high-scoring articles, it is necessary to have some data in the body to prove that there are many animal models of liver cancer, and the construction difficulty is also different. The following is a summary of various methods:

Third, the in vitro and in vivo models have been selected, then what kind of lentiviral vector does my experiment need to use to manipulate the gene?

Promoters, fluorescent markers, and resistance tags are the three major factors we need to consider when choosing a carrier. Different components also require corresponding changes in the experiment. For liver cancer research, hAAT1 and ALB are the more commonly used liver tissue-specific promoters. According to the example of a god figure, customizing your exclusive carrier is as simple as that!

Shanghai Jikai Gene Chemical Technology Co., Ltd. was established in 2002 and has a BSL-2 level lentivirus packaging laboratory. The virus production line has passed the ISO9001 quality management system verification, and the monthly average custom genetic virus product packaging has exceeded 1000 times. Lentiviral production uses six QC assays, viral purity fractionation and absolute quantitative detection of viral titers to ensure virus quality.

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PS: Friends who want to know more about the Tumor Tools Guide can pay attention to the "Jikai Gene" WeChat public number, and more exciting articles waiting for you.

references

[1] Li, Xiaolei, et al. "Overexpression of Bmi-1 contributes to the invasion and metastasis of hepatocellular carcinoma by increasing the expression of matrix metalloproteinase (MMP)-2, MMP-9 and vascular endothelial growth factor via the PTEN/ PI3K/Akt pathway." International journal of oncology 43.3 (2013): 793-802.

[2] Zhang, Ke-Zhi, et al. "Arsenic trioxide induces differentiation of CD133+ hepatocellular carcinoma cells and prolongs posthepatectomy survival by targeting GLI1 expression in a mouse model." Journal of hematology & oncology 7.1 (2014): 1.

[3] Sakr, Hany I., et al. "Characterization of an Oncolytic Adenovirus Vector Constructed to Target the cMet Receptor." Oncolytic virotherapy 4 (2015): 119.

[4] Xiao, Jianbiao, et al. “The association of HMGB1 gene with the prognosis of HCC.” PLoS One 9.2 (2014): e89097.

[5] Peng, Chuanhui, et al. “A critical role for ZDHHC2 in metastasis and recurrence in human hepatocellular carcinoma.” BioMed research international 2014 (2014).

[6] Jin, Wei, et al. "Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine1." Acta pharmacologica sinica 28.6 (2007): 859-868.

[7] Hao, Chun‐Qiu, et al. “Role of Nogo‐A in the regulation of hepatocellular carcinoma SMMC‐7721 cell apoptosis.” Molecular medicine reports 9.5 (2014): 1743-1748.

[8] Chai, Zong-Tao, et al. "MicroRNA-26a inhibits angiogenesis by down-regulating VEGFA through the PIK3C2α/Akt/HIF-1α pathway in hepatocellular carcinoma." PloS one 8.10 (2013): e77957.

[9] Wang, Fei, et al. "RASSF10 is an epigenetically inactivated tumor suppressor and independent prognostic factor in hepatocellular carcinoma." Oncotarget 7.4 (2016): 4279.

[10] Luo, Zai-Li, et al. "A splicing variant of Merlin promotes metastasis in hepatocellular carcinoma." Nature communications 6 (2015).

[11] Yang, Xiaoming, et al. "Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo." PloS one 8.4 (2013): e60853.

[12] Ng, Kevin TP, et al. "A garlic derivative, S-allylcysteine ​​(SAC), suppresses proliferation and metastasis of hepatocellular carcinoma." PLoS One 7.2 (2012): e31655.

[13] Yu, Song-Tao, et al. "An optimized telomerase-specific lentivirus for optical imaging of tumors." Cancer research 70.7 (2010): 2585-2594

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