Heavy building is one of the important Chinese herbal medicines in China. Due to the relatively small planting area of ​​heavy buildings and the large demand for heavy buildings, the heavy building is still in short supply. It can be said that there is a very good planting value. In terms of medicinal resources, special tissue culture and rapid propagation technology, I hope that everyone can help. (1) Material selection and disinfection 1. material selection The explants of the tissue culture in the re-cultivation should choose the rhizome which is vigorously grown in the leaf-expanding stage as the explant, because the callus of the rhizome is the highest in the induction of explants, up to 16.6%, and the next time in the germination period, In the corolla whitening stage and the inverted seedling stage, it is difficult to induce callus formation in the roots. 2. disinfection After sampling the heavy building, disinfection measures should be taken. Soaking with 200mg/L carbenicillin sodium or cefazolin sodium for 5 hours. When soaking, it should be oscillated (26°C, 112r/min). After washing out, wash with sterile water for 2 times → rinse with 75% ethanol for 30 seconds → wash with sterile water for 1 time → lg/L mercury for 5 minutes → wash with sterile water for 3 times → dry and cut into 0.5 The size of cm3 is → after inoculation on the medium. (2) Medium selection and culture conditions 1. Medium MS medium was used as the basic medium, different mediums were selected according to different explants, and different kinds and concentrations of hormones were added as needed to induce the formation of bulbs, bud differentiation and rooting medium. 2. Culture conditions When the culture is carried out, the temperature of the culture chamber is required to be in the range of 20 degrees, and the illumination time is 16 hours. (3) Bud differentiation is the rooting seedling culture The callus was transferred to the proliferation and differentiation medium, and the medium was changed once every 90 days, and the total of the medium was changed 3 times. The callus pieces gradually changed from pale yellow to white, and the surface gradually changed from rough to smooth. After about 30 days, it will gradually differentiate into buds. Differentiation shoots, if rooting induction is not carried out, continue to grow on the proliferation and differentiation medium for about 150 days to form intact rootless shoots. 210 to 240 days is a proliferative cycle, the multiplication coefficient is 1-2 times, and the differentiated buds are inoculated on the rooting medium. During the cultivation, the base of the buds will gradually brown and elongate into rhizomes, and culture for 60 days. Left and right, 2-3 roots can grow at the base of the bud. (4) Refining seedling transplanting Remove the buds with a root length of 2-3 cm, wash the medium and transplant the buds into the humus soil, and put them in an environment with a temperature of 18-20 degrees, while maintaining the soil moisture of 50%-60%, about After 180 days, the buds will be unearthed to grow leaves, and then complete plants will be formed. The survival rate of the unearthed leaves will reach 65%. Household Gloves,Gloves Microfiber,Flock Lined Glove,Household Latex Work Glove ZHANGJIAGANG DINSHENGLIN TRADING CO.,LTD , https://www.dslhouse.com
