Plant tissue culture media includes a variety of ingredients: inorganic salts, vitamins, amino acids, growth regulators, sugars, agar (or gellan gum), and water. All of these ingredients have at least one or more effects in plant tissue culture . Order number A0202 C0249 E0208 K0215 English name CHÉE & POOL medium including vitamins Eriksson (ER) medium including vitamins Knudson C Orchid medium Chinese name Anderson's medium contains vitamins CHÉE & POOL medium contains vitamins Eriksson (ER) medium contains vitamins Knudson C Orchidaceae Medium Base Salt Large number of elements NH 4 NO 3 mg/L 400 1650 1200 500 (NH 4 ) 2 ·SO 4 mg/L 500 CaCl 2 mg/L 332.02 332.02 MgSO 4 mg/L 180.54 180.54 180.54 122.15 KH 2 PO 4 mg/L 170 340 250 NaH 2 PO 4 mg/L 330.6 KNO 3 mg/L 480 1900 1900 Ca(NO 3 ) 2 mg/L 492.3 241.3 KCl 250 Trace element H 3 BO 3 mg/L 6.2 6.2 0.63 CoCl 2 .6H 2 O mg/L 0.025 0.025 0.025 CuSO 4 .5H 2 O mg/L 0.025 0.025 0.025 FeSO 4 .7H 2 O mg/L 25 FeNa-EDDHA 73.4 36.7 36.7 MnSO 4 .H 2 O mg/L 16.9 0.85 1.69 5.68 Na 2 MoO 4 .2H 2 O 0.25 0.25 0.025 KI mg/L 0.3 ZnSO 4 .7H 2 O mg/L 8.6 8.6 9.15 Vitamins (organic ingredients) Glycine mg/L 2 Inositol mg/L 100 10 Niacin mg/L 1 0.5 Pyridoxine hydrochloride mg/L 1 0.5 Thiamine hydrochloride mg/L 0.4 1 0.5 Additional ingredients Adenine sulfate mg/L 80 Mg (powder) / L medium 2009.26 4458.49 4004.42 1894.13 Order number L0230 N0224 N0252 English name Linsmaier & Skoog medium Nitsch medium including vitamins NLN medium Chinese name LS medium Nitsch medium contains vitamins NLN medium base salt Large number of elements NH 4 NO 3 mg/L 1650 720 CaCl 2 mg/L 332.02 166 MgSO 4 mg/L 180.54 90.27 61 KH 2 PO 4 mg/L 170 68 125 KNO 3 mg/L 1900 950 125 Trace element H 3 BO 3 mg/L 6.2 10 10 CoCl 2 .6H 2 O mg/L 0.025 0.025 CuSO 4 .5H 2 O mg/L 0.025 0.025 0.025 FeNa-EDDHA mg/L 36.7 36.7 36.7 MnSO 4 .H 2 O mg/L 16.9 18.94 18.95 Na 2 MoO 4 .2H 2 O mg/L 0.25 0.25 0.25 KI mg/L 0.83 ZnSO 4 .7H 2 O mg/L 8.6 10 10 Vitamins (organic ingredients) Glycine mg/L 2 Inositol mg/L 100 100 Niacin mg/L 5 Pyridoxine hydrochloride mg/L 0.5 Thiamine hydrochloride mg/L 0.4 0.5 Biotin mg/L 0.05 Folic acid mg/L 0.5 Mg (powder) / L medium 4402.49 2178.74 386.95 Fish Collagen,Fish Collagen Powder,Fish Collagen Granules,Cod Skin Collagen Ningbo Nutrition Food Technology Co.,ltd. , https://www.collagenworkshop.com
Mineral elements in plant tissue culture media are used to synthesize organic molecules or act as catalysts in enzymatic reactions after entering plant cells. Soluble salt ions play an important role as counter ions in the transport of plant ionized molecules, regulation of osmotic pressure and maintenance of the electrochemical potential of plants.
Nitrogen, sulfur and phosphorus are components of proteins and nucleic acids. Magnesium ions and many trace elements are essential components of many enzymes and organelles and therefore play an important catalytic role in a variety of reactions. Calcium and boric acid are mainly present in the cell wall of plants, especially calcium ions play an important role in maintaining biofilm stability. In contrast, potassium ions and chlorides play an important role in osmotic adjustment, participating in maintaining the balance of the electrochemical potential, and are also activators of many enzymes.
In addition to the commonly used MS medium, B5 medium, woody plant medium and N6 medium, Duchefa also provides some other plant tissue culture media according to different species, including: Anderson's medium, CHÉE & POOL medium, CLC CP /EP medium, De Greef & Jacobs medium, DKW medium, Eriksson (ER) medium, Gresshoff & Doy medium, Heller medium, KAO & Michayluk medium, Knudson C orchid plant medium, Lindemann Orchidaceae Plant medium, LS medium, Litvay medium, Nitsch medium, NLN medium, Orchimax medium, Quoirin & Lepoivre medium, Rugini medium, S medium, Schenk & Hildebrandt medium, Vacin & Went medium ( VW medium), Westvaco WV5 medium and White medium are available for customers to choose.
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