Identification of burdock traits

Character identification

Achenes are long and obovate, flat at both ends, slightly curved and slightly curved, 5-7 mm long and 2-3 mm in diameter. The surface is gray-brown or grayish-brown, with many fine dark spots and obvious longitudinal ridges. The apex is wider, with a ring, and the center is slightly studded with streaks of style; the base is narrow and has a rounded pedicel. Hard, broken after the cotyledons can be seen two, pale yellow and white, rich in sex. The fruit is odorless; the seeds are gas-specific, bitter and slightly pungent, and slightly numbed.

It is better to use large, full, and gray-brown colors.

Microscopic identification

Achenes cross-section: The outer skin is a series of square-shaped parenchyma cells of varying sizes, with curved walls and multiple ruptures; the outer layer is stratum corneum. The mesocarp is not uniform in thickness, the cell wall is thicker, brownish or dark brown, and slightly woody; small vascular bundles are often found at the ridge. The inner endocarp is narrow, with a brown-yellow depleted cell layer. The cell boundary is unclear and is filled with 1 column of calcium oxalate crystals. The outermost seed coat is a shed-producing primordial cell, which is distorted and closely arrayed, with a length of 75-120 μm, a diameter of 10-30 μm, a very thick wall, and a stratified lining. The nutrient layer is a series of parenchyma cells and is often unclear. Number of endosperm cells, containing fatty oils. The cotyledon cells are full of maggots and fatty oils, and contain tiny clusters of calcium oxalate crystals, occasionally small square crystals.

Physical and chemical identification

(1) Take 2g of powdered product of degreasing, add 20ml of ethanol and warm immerse for 1 hour, and filter. The filtrate was used for the following tests: 1 Take 2ml of the filtrate and add 1% aluminum chloride in ethanol to give a blue-green color. (Check lignans) 2 Take 2ml filtrate, add an equal volume of 3% sodium carbonate aqueous solution, boil on the water bath for 3-5min, let cool, add diazotization reagent, the solution is red. (Check lignans)

(2) Take 5 g of coarse powder and add 10 ml of dilute aqueous hydrochloric acid (pH 1.0-2.0). Soak overnight and filter. Take 3 ml each of the filtrate 2 ml, set 3 test tubes, respectively, plus iodide mercury and potassium reagents, potassium iodide reagents, silicotungstic acid reagent 1 drop, respectively, then produce white, brown red and white precipitate. (check alkaloids)

(3) take a small amount of the product powder, set the UV lamp (365nm) under observation, significant green fluorescence.


PCR tube

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