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Discussion on the Advantages and Disadvantages of Spectrophotometry and Measurement in Pipette Calibration (Part I)
Discussion on the Advantages and Disadvantages of Spectrophotometry and Measurement in Pipette Calibration (Part I)
Before we start discussing this topic, we should first briefly understand what a pipette is, what its function is, and what factors affect its calibration during the calibration process.
Pipettes, as the name suggests, are instruments that can perform liquid transfer functions. Of course, current pipettes not only enable liquid transfer, but also enable accurate metering during transfer. Therefore, pipette calibration It is the test of its basic functions and measurement results. The liquid transfer function of the pipette can be judged by visual observation, but the results of the measurement need to be detected and verified by the method to be discussed in this paper.
At present, the mainstream or main method for the detection and verification of pipette measurement results is the measurement method, sometimes referred to as the “weighing methodâ€. It is commonly referred to as the “weighing method†because the principle of the method is to weigh a certain volume of liquid metered by the pipette by an electronic balance, and based on this data, the actual measurement result of the pipette is inferred. value. Based on this principle, it is obvious that the pipette calibration process is extremely susceptible to the following factors.
1 temperature, because the volume of the liquid is subject to temperature changes, therefore, a certain mass of liquid, at different temperatures, its volume is also different, which leads to the use of pipettes to measure a certain volume of liquid, The balance reading will be degraded due to the increase in temperature, so that the capacity of the pipette inferred from this result will be less than the actual capacity;
2 Atmospheric pressure, the volume of the same mass of liquid is different under different atmospheric pressures, but usually the difference is very slight or even negligible;
3 The levelness and stability of the high-precision electronic balance used for weighing and the position of the weighed items placed on the electronic balance tray will affect the weighing result;
4 the impact of the operating habits of the operator;
5 measuring instruments such as electronic balances, thermometers and other errors caused by their own;
6 The relative humidity of the environment affects the liquid with the measurement;
It can be seen that the calibration of pipettes by traditional measurement methods will be affected by various factors such as environment, personnel and measuring instruments/equipment. For this reason, many pipette calibration laboratories have to The various factors that influence the calibration results are designed to control and control the laboratory environment, which minimizes the impact of these factors. But doing so will lead to a new, practical problem. Is the calibration data obtained in a professional calibration laboratory suitable for the actual use of the pipette? That is, can these calibration reports or verification reports issued by professional laboratories truly reflect the performance of the pipettes in the end user? Because the environment at the end user is very different, some even higher than these professional laboratories, but more are far lower than these professional laboratories, then the data obtained by these professional laboratories, It is impossible or difficult to get a reproduction at the end user.
In fact, it is also true! In many cases, testing a qualified pipette in a professional laboratory, when the end user recalibrates at his workplace, finds that the results are not ideal. This is not a problem with the pipette itself, actually because The location of the test has changed, the environment has changed, and the various factors that affect the calibration results have changed! The most effective way to solve this problem is to go directly to the end user's actual use place to calibrate the pipette used by the user, so that the calibration result is the most effective, for a specific user, It is the most authentic!
To this end, we can of course relocate the professional laboratory equipment, such as high-precision electronic balances, professional technicians, environmental control and other equipment to the end user for calibration, but this is obviously uneconomical and does not work. of. Because some instruments, such as high-precision electronic balances, once they have left the original fixed place of use, they need to be re-verified before they can be used. So is there another way to solve this problem efficiently and simply? This is a new pipette calibration method ____ spectrophotometry to discuss with you!
What is spectrophotometric calibration?
See "Discussion on the Advantages and Disadvantages of Spectrophotometry and Measurement in Pipette Calibration (Part II)"