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Test principle
a-Hydroxybutyrate dehydrogenase (a-HBD) catalyzes the reversible reaction:
The reaction is monitored spectrophotometrically by measuring the decrease in NADH at 340 nm, and the decreasing rate of absorbance is proportional to a-HBD activity.
Product Features
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Linear deviation ≤ ± 10.00%
Technical Parameters
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Temperature
37ºC
Optical path length (OPL)
1.0 cm
Main wavelength
340 nm
Secondary wavelength
380 nm (Optional)
Method
Velocity method
Reaction direction
Negative
Sample
5 μL
R1
200 μL
R2
50 μL
Delay time
100 s
Reaction time
180 s
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Packing Specifications
R1: 2×60 mL, R2: 2×15 mL
R1: 4×60 mL, R2: 4×15 mL
R1: 1×40 mLl, R2: 1×10 mL (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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The a-Hydroxybutyrate dehydrogenase (a-HBD) assay kit is intended for the accurate and direct quantitative measurement of a-HBD activity in human plasma.
Test principle
a-Hydroxybutyrate dehydrogenase (a-HBD) catalyzes the reversible reaction:
The reaction is monitored spectrophotometrically by measuring the decrease in NADH at 340 nm, and the decreasing rate of absorbance is proportional to a-HBD activity.
Product Features
Â
Linear deviation ≤ ± 10.00%
Technical Parameters
Â
Temperature
37ºC
Optical path length (OPL)
1.0 cm
Main wavelength
340 nm
Secondary wavelength
380 nm (Optional)
Method
Velocity method
Reaction direction
Negative
Sample
5 μL
R1
200 μL
R2
50 μL
Delay time
100 s
Reaction time
180 s
Â
Â
Packing Specifications
R1: 2×60 mL, R2: 2×15 mL
R1: 4×60 mL, R2: 4×15 mL
R1: 1×40 mLl, R2: 1×10 mL (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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Alpha-Hydroxybutyrate Dehydrogenase (alpha-HBD) Assay Kit/Test Kit/Ivd Reagent/Diagnostic
Model NO.: alpha-HBD
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 ml, R2: 2*15 mL
Origin: Jinan, Shandong
Model NO.: alpha-HBD
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 ml, R2: 2*15 mL
Origin: Jinan, Shandong
The a-Hydroxybutyrate dehydrogenase (a-HBD) assay kit is intended for the accurate and direct quantitative measurement of a-HBD activity in human plasma. 1. Linear range of a-HBD activity: [3, 800] U/L
Linear correlation coefficient ∣r∣≥ 0.9902. Absorbance of blank ≥ 0.90
3. Changing rate of absorbance of blank ΔA/min ≤ 0.02 Â
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.03 while testing the specific a-HBD sample of activity of 274 U/L.5. Accuracy
Relative deviation is within ±10%.6. Precision
Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.1. Linear range of a-HBD activity: [3, 800] U/L
Linear correlation coefficient ∣r∣≥ 0.9902. Absorbance of blank ≥ 0.90
3. Changing rate of absorbance of blank ΔA/min ≤ 0.02 Â
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.03 while testing the specific a-HBD sample of activity of 274 U/L.5. Accuracy
Relative deviation is within ±10%.6. Precision
Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.