Polygonatum is a medicinal plant that can treat spleen and stomach weakness, lung and phlegm, red leucorrhea and so on. At present, Huang Jing is mainly based on rhizome reproduction. The following small series will take you to understand the research progress of Huang Jing's breeding technology. 1 Rhizome's rhizome breeding technology In the late autumn or early spring around the end of March, select the roots and stems of the 1-2 year old plant with no pests and diseases, select the young part of the apex, cut into several sections, each section has 3-4 sections, after the incision is slightly dried and collected, According to the row spacing 20-24cm, plant spacing 10-16cm, depth 5cm planting, after the soil is slightly suppressed and watered, after every 3-5d watering once to keep the soil moist. When planting at the end of autumn, cover some fat and grass to keep warm after freezing. Tian Qijian et al. The yellow sperm clonal 1 year old seedlings of the Huangjing GAP test demonstration base are divided into 4 grades according to the bud length, root number and root length. It is stipulated that grades 1, 2 and 3 can be used for planting, and grade 4 can not be used as seedlings. It is recommended that the 1-year-old seedlings should be transplanted in December of the second year of nursery. Zheng Yunfeng and Li Songtao also reported that Huangjing retains the best roots. In order to ensure the wild efficacy of Huang Jing, it requires a large number of wild germplasm that grows vigorously for 2-3 years, which causes great damage to the wild Huangjing resources in China and affects sustainable development. 2 Tissue culture of Polygonatum Tissue culture is a very effective means of rapid propagation. Its application technology is also relatively mature, and its application in medicinal plants has become more extensive, providing a new way for the protection of endangered medicinal plant resources and the production of alternatives. By applying this method, a large number of seedlings can be obtained in a short period of time, and polyploidy variation may occur, thereby opening up a way for creating excellent new varieties. In the current research, callus induction was carried out by its roots and seeds, and the application of bud root lotus was the most widely used. A lot of studies have been done on callus induction medium, rooting medium and regeneration medium of plants, which indicates that 6-AB has significant effect on the growth of callus induced by Polygonatum, but the optimal medium is not the same. Polygonatum is mainly propagated through root ridges, and the reproductive coefficient is low, which cannot provide a large number of provenances. In addition, long-term dependence on asexual reproduction is likely to cause deterioration of germplasm traits. One of the benefits of tissue culture technology is that it can greatly alleviate the dependence of production on wild germplasm resources, and can ensure the wild efficacy of Huangjing, which is conducive to the large-scale cultivation of Polygonatum. Another advantage is the tissue culture and cultivation of virus-free seedlings, which is conducive to preventing the occurrence of yellow sperm pests and diseases. 3 Huang Jing's seed reproduction Zhang Yucui et al. studied the germination rate of Huangjing seeds under natural conditions and had a comprehensive dormancy phenomenon. The main cause of dormancy is the physiological post-ripening of the embryo; the seed is difficult to absorb water due to the impermeability of the seed coat; the endosperm cells of the seed are small, the cytoplasm is dense and closely arranged, the interstitial space is small, and the symplast transport of the substance is affected; The seed contains a germination inhibitor or the like. Since there are currently few applications for the propagation of Polygonatum seeds, there are fewer successful applications. According to Yang Zilong et al., the use of seed propagation treatment by Huang Jing is cumbersome, the emergence time is longer, the emergence rate is not high, and the cost is high, which is not conducive to large-scale reproduction of Huang Jing. Zhao Zhi and other research results show that the harvest time of Huangjing fruit is generally in mid-November; the harvesting period of Rhizoma Polygonatum and Scutellaria Baicalensis in late October, the emergence rate of Huangjing is higher. The seeds of the yellow essence are treated by fermentation and rinsing to obtain seeds, and it is recommended to use low temperature sand and frozen sand to preserve the seeds. Storage for about 100d (from late February to early March of the next year) to start seedling raising, seedling method: use fine and uniform sandy loam to pave the germination bed, draw a 2cm sulcus according to the line spacing of 15cm, rinse the seeds with water for 12h. After being placed in a fine groove of the seedbed according to the specification of 5cmx5cm, the fine soil beside the fine groove is covered, and the raft is compacted with a raft. The water is poured once, and a thin plastic straw shed is covered with a thin straw. Wu Weichun and other researches show that during the breeding process of Huangjing seeds, the fresh seeds harvested in the same year are planted in large greenhouses in the greenhouse, and the indoor temperature is controlled at 25 °C-27 °C, in late January of the next year, The seedling trays are moved to the outside, and the natural low-temperature freezing is carried out outside. In April, the seedlings are all unearthed. From the end of July to the beginning of August, the ground part is withered, and the underground secondary rhizomes are all formed. At this time, all the rhizomes are taken out and transplanted to the field. . In this way, it is possible to emerge one year earlier than natural conditions and shorten the cultivation time. The above is the whole content of the rhizome propagation technique of Rhizoma Polygonati. At present, there are not many reports on the breeding of Huangjing seed in China. You can learn to refer to this rhizome propagation technique. 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