Microscope oil mirror use:

1. In the use of oil mirrors, when the observation area is selected with a low-powered objective lens, people tend to underestimate the small object field of the high-magnification objective lens, so that it is possible to focus on a null field. At the same time, the center of the objective lens is rarely extremely accurate, so the field of view of the high power objective cannot be estimated very accurately. These bring great difficulty to the focus of the oil mirror. In this case, a very small piece of cigarette paper or a very thin tin foil can be sealed with the specimen under the coverslip, so that it is easy to use the oil mirror to find the focal plane that is very close to the specimen.

2. When using the oil mirror, you can directly turn back to the low magnification objective to observe without immersing the oil. The oil immersion layer does not have a great influence on the formation of the image when the low magnification objective lens is used, and the front lens of the objective lens does not touch the oil immersion, and the immersion oil on the cover glass does not increase the coverage of the cover glass. influences.

3. The earliest immersion oil used in Olympus microscopes is fir oil with a refractive index of 1.512-1.518. This immersion oil has increased dryness and slower polymerization characteristics when exposed to air. Many microscopy manufacturers now offer a non-resin synthetic immersion oil with a refractive index of 1.5 1 5-1.5 18 and suitable dispersion characteristics. Due to its good properties and suitability for high quality oil immersion work, The above two resinous immersion oils have been gradually replaced. Therefore, it is recommended to use this type of mirror oil when using oil mirrors.

4. It takes only one drop to soak the oil. Excessive oil immersion is not only unhelpful for observation, but also causes a lot of trouble for the cleaning of the oil mirror after use. At the end of the observation, even if non-resin-based immersion oil is used, carefully clean the objective lens and the cover glass. First wipe off the oil with a dry photographic paper or a dry cloth, and then use xylene or gasoline. Rub the mirror paper or cloth to wipe off the remaining oil.
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