Leading the industry standard for microplate inspection systems

After 13 years of development, the SpectraMax® M series of multi-microplate readers have become the industry standard leader due to their excellent inspection performance, reliable performance and the trust of researchers. Easily upgrade to meet your inspections based on your application needs. Instrument setup and data processing are all done with the help of professional SoftMax® Pro software, all with three-function cuvette detection slots, temperature control, and microplate oscillations. Professional verification documents and a rigorous verification process ensure that each instrument software and hardware meets the requirements.

The power of SpectraMax M

More than 20,000 published papers have been applied to the SpectraMax M Series Multi-Purpose Reader. M Series microplate readers support UV and visible light absorption analysis and fluorescence intensity detection of microplates and cuvettes, as well as UV and UV absorption, fluorescence, glow chemiluminescence, fluorescence polarization, TRF and Detection of HTRF. Choosing the right combination of features will help you with your research.

All M Series Microplate Readers support cuvettes and 6 to 384 well microplates. Other standard advantages include spectral scanning in 1 nm steps, detection in up to 6 wavelengths in one read in light absorption mode, and detection of up to 4 wavelengths in the next reading in other modes.

- Patented automatic PMT optimization system
- Patented PathCheck sensor
- Comprehensive IQ/OQ verification service for GLP/GMP labs
- Verification board for light absorption, fluorescence and chemiluminescence modes
- Easy integration automation
- Provide matching test kits

reliability

In May 2011, with its durability, the Molecular Devices SpectraMax M5e became the first microplate reader to enter space. NanoRacks added it to the commercial hardware experimental platform on the International Space Station, providing scientists with the opportunity to perform microplate inspection in a microgravity environment. In order to better operate in the weightless environment of the space station, MolecularDevices' most reliable and feature-rich SpectraMax M5e has been further optimized and optimized, including temperature control functions and support for ground stations using the industry-leading data acquisition and analysis tool SoftMax Pro for remote setup. Experimental parameters.

On July 18, 2016, NanoRacks again introduced the optimized SpectraMax M5e microplate reader to the International Space Station via SpaceX Commercial ResupplyMission-9.

Automatic instrument verification

SpectraTest Series Verification Board

The SpectraTest® ABS1, FL1, and LM1 verification kits from Molecular Devices provide automated, comprehensive and traceable optical performance verification, as well as automated calibration of our microplate readers.

- NIST traceability
- Recertification by our ISO 17025 accredited laboratory
- Light absorption verification (ABS1)
- Fluorescence verification (FL1)
- Chemiluminescence verification (LM1)
- Inspection automation
- Pre-defined verification templates are available in SoftMax Pro software

IQ/OQ/PM service

The compliance process for Molecular Devices microplate readers in GLP or GMP environments is now more reliable, safer and more convenient. The now-compliant, compliant, electronic IQ/OQ/PM service is Molecular Devices' unique verification solution that preserves traditional compliance service documents in a secure electronic form and is remotely accessible.

Compliance instrument verification

During each field validation process, trained Molecular Devices hardware engineers will perform calibration and electronic recording instrument operations in accordance with IQ/OQ requirements. All calibration and periodic maintenance programs automate the process of each verification through automation while ensuring the integrity of data and results analysis. Our services include:

- Inspection and documentation by installation verification (IQ) All materials necessary for installation have been received and properly installed in accordance with Molecular Devices ISO 17025 certification.
- Detect the mechanical, electrical and optical components of each instrument by operational verification (OQ) and verify that the instrument will operate as intended by the manufacturer.
- Preventive Maintenance (PM) ensures that each instrument meets operational requirements through comprehensive multi-point inspection. By proactively addressing potential hazards, PM ensures that each instrument remains in optimal operating condition.

GxP compliance

SoftMax Pro GxP Compliance Software extends our industry-leading data acquisition and analysis solutions to laboratories in GMP, GLP, 21 CFR Part 11 or similar environments to ensure the safety of electronic records.

Safe, traceable electronic record keeping

- Control user access with precise permissions architecture and unique entry information
- Electronic signature support verification, authorization and approval operations
- Audit trail file providing user operation history for each data file

Economic fast

- We offer a wealth of verification tools that reduce time and economic costs by up to 50% compared to collecting and analyzing data using multiple inspection platforms
- From detection to analysis to verification we provide uninterrupted data monitoring that is trustworthy
- SoftMax Pro 7 GxP is our fourth generation support compliance software
- The world's top 50 pharmaceutical companies use our software
- Local and remote direct definition of user information

Software verification

For researchers working in GLP or GMP laboratories, the SoftMax Pro software verification program provides the most comprehensive documentation and tools available to validate GxP administrator functions, software operation and analysis capabilities.

- Verification time reduced from 6 months to 3 days supporting parallel line analysis, 4-P and 5-P curve fitting verification
- Comprehensive and complex testing for routine experimental calculations
- Instantly available data for OQ verification testing
- Printable IQ/OQ data for GLP/GMP written records

MycoAlert Mycoplasma Test Kit Application

Mycoplasma is currently the smallest known prokaryote and is a common source of contamination in mammalian cell culture. Mycoplasma contamination puts a lot of cost to cell culturers because they alter the morphology, vitality, and metabolic characteristics of contaminated cells. Mycoplasma is difficult to find in the medium by a microscope. At present, the pollution for this situation requires a sensitive and reliable detection method. The traditional methods for detection of mycoplasma contamination, such as time-consuming labeling and cumbersome PCR, are difficult to obtain accurate analysis.

The MycoAlert Mycoplasma Test Kit and MycoAert PLUS Test Kit are from LonzaWalkersville Co., Ltd. This reagent is based on a biochemical reaction coupled with a luminescent microplate reader to detect certain mycoplasma enzyme activities. The presence of these enzymes provides a rapid screening process that allows very sensitive detection of mycoplasma contamination of test samples.

Detection of fluorescent protein expression in living cells using "non-invasive" technology

Fluorescent proteins play an increasingly important role in the monitoring of in vivo biological studies. These fluorescent proteins are stable, have little cytotoxicity, and produce visible fluorescence in the body without additional assistance. The microplate reader with fluorescence function will be a better, more convenient and more efficient option. Here, we will show how the Molecular Devices microplate reader can use the "non-invasive" technology to detect the activity. Cellular fluorescent protein.

Detection of cell proliferation using the SpectraMax microplate assay system and the CyQUANT kit

Quantification of cell proliferation by fluorescence can be used to quickly and easily detect the effects of drugs or other experimental treatments on cell growth. Life Technologies' CyQUANT Cell Proliferation Kit is a highly sensitive, reproducible and simple method for performing fluorescent microplate assays.

The GR dye of CyQUANT can be labeled on the nucleus and the number of cells counted by fluorescence. The ratio of DNA to RNA changes with the cell cycle. Therefore, the CyQUANT kit can be used as a standard curve by RNase lysate and nucleic acid amount.

This application note describes how to use the CyQUANT kit and Molecular Devices' SpectraMax microplate assay system and SoftMax® Pro software to detect cell proliferation. Two methods were introduced, one is a cell-based method, and the other is to prepare a DNA standard curve by dissolving a cell sample with RNase.

IMAP kinase phosphorylation detection kit application

The principle is that the detection reagent is mixed with special metal nanoparticles, which can specifically bind specifically to the phosphate ion group on the substrate. IMAP technology is not a specific antibody-based technology, but a general technology platform. Used in the detection of any kinase, phosphatase and phosphodiesterases. After the fluorescently labeled substrate is catalyzed by the enzyme molecule, the IMAP binding system is added to terminate the reaction and bind to the phosphate system. As the corresponding reaction occurs, the substrate will bind to the microspheres. We use FP and TR-FRET functions. The microplate reader is tested accordingly.

High-throughput complex inflammation analysis using primary cells

An important step in the inflammatory response is the specific binding of cell surface antigens to immune cells in blood vessels. Therefore, for the timely monitoring of these molecular changes, such as VCAM, E-selectin, and HLADR on endothelial cells, we use multi-channel fluorescence to read the inflammatory markers on the surface of human primary cells, and evaluate different media on this basis. The regulation of the inflammatory response.

HTRF cAMP HiRange test

HTRF® is a versatile technology developed by Cisbio Bioassays to detect biomolecular interactions. This technology combines fluorescence resonance energy transfer (FRET) technology with time-resolved (TR) fluorescence to eliminate short-lived background fluorescence. Here, we show how to use the SpectraMax microplate reader for reliable, high-throughput HTRF detection with a high degree of repeatability for perfect Z' factor and EC50 values.

The HTRF preset experimental template that comes with SoftMax Pro software greatly simplifies data acquisition and analysis.

HTRF cAMP Dynamic 2 and IP-One detection

The HTRF-certified SpectraMax M5e multi-function microplate reader enhances the flexibility of the dual monochromator system and multi-mode detection capabilities, extending the range of detection methods for researchers. When tested on the SpectraMax M5e reader using IP-One and cAMP HTRF from Cisbio, the results showed a very wide dynamic range and a perfect Z' factor ranging from 0.78 to 0.91. The HTRF preset experimental template that comes with SoftMax Pro software greatly simplifies data acquisition and analysis.

Quant-iT PicoGreen dsDNA experiment

Double-stranded DNA is typically quantified by measuring the 260 nm absorbance of the DNA solution using a microplate reader. However, this method typically only detects a minimum concentration of 250 ng/mL on a microplate reader. For biological applications involving small volume samples, such as next-generation sequencing and amplification product quantification, a more sensitive approach can be met. Life Technologies' Quant-iTPicoGreen dsDNA assay is more specific for DNA and 1000 times more sensitive than traditional light absorption methods. The product information is nominally performed on a microplate using a single dye concentration with a dynamic range of 250 pg/mL to 1000 ng/mL. This application note demonstrates the ability to stably detect double-stranded DNA as low as 100 pg/mL using Molecular Devices' SpectraMax® Fluorescence Microplate Reader and Quant-iT PicoGreen assay.

Use the NanoOrange Protein Kit

Traditional colorimetric methods, such as the 280 absorbance, BCA, Bradford or Lowry methods, have poor sensitivity when using the microplate format. The NanoOrange® Protein Quantitation Kit from Life Technologies has a dynamic range of 10 ng/mL to 10 μg/mL in a multiwell plate format. The data presented in this application note confirms the dynamic range and minimum detection limit of the NanoOrange kit. The NanoOrange experiment is a fast and sensitive method for detecting proteins when used with the Gemini XPS microplate reader or other SpectraMax fluorescent microplate readers using SoftMax Pro software. The analytical capabilities of SoftMax Pro software provide experimental quantification in an easy-to-read, customer-customizable reporting format.

APIs & Intermediates

Intermediates of Cladribine, Carvedilol, Lurasidone, olmesartan, Risedronate Sodium, Atazanavir, Saxagliptin, Dabigatran,Dapoxetine,Cefixime,Ceftaroline fosamil and etc.

In the short span of time, we have emerged as most promising pharmaceutical intermediates manufacturers, chemical intermediates and bulk drug intermediates suppliers. Our consistent supply, quality products and dedication towards clients have opened up many international avenues for our growth.

In addition, the company also can follow the customer's product needs custom synthesis services

MAIN API PRODUCTS USP/BP

PRODUCT NAME	CAS NUMBER	SPEVIFICATION
Azithromycin	117772-70-0	BEP
Cefpirome Sulphate sterile	84957-29-9	USP JP16
Ceftriaxone Sodium (Sterile)	104376-79-6	USP31
Cefotaxime	64485-93-4	USP30
Ciprofloxacin HCL	85721-33-1	USP/BP
Gentamicin sulphate	1405-41-0	BP
Levofloxacin	100986-85-4	USP27
Lincomycin Hydrochloride	859-18-7	EP6.0
Moxifloxacin Hydrochloride	186826-86-8	USP31
Tigecycline	220620-09-7	USP
Linezolid	165800-03-3	EP
Dexamethasone	50-02-2	USP/BP/EP
Methylprednisolone	83-43-2	USP/BP/EP
Dexketoprofen trometamol	156604-79-4	BP2008
Ibuprofen	15687-27-1	BP
Metamizol	68-89-3	DAB
Sulindac	38194-50-2	USP/BP/EP
Naproxcinod	163133-43-5	USP28
Tripelennamine Hydrochloride	154-69-8	USP28
Itraconazole	84625-61-6	USP/BP
Cytarabine	147-94-4	USP31
Leucovorin Calcium	1492-18-8	USP32
Valsartan	137862-53-4	USP30
Telmisartan	144701-48-4	USP31
Rosuvastatin Calcium	147098-20-2	USP/BP
Pitavastatin Calcium	147526-32-7	USP/BP
Fluvastatin	93957-54-1	USP31
Vinpocetine	42971-09-5	EP6.0
Atazanavir	198904-31-3	BP
Rosiglitazone	122320-73-4	USP30
Esomeprazole Magnesium	161973-10-0	USP/BP
Topiramate	97240-79-4	USP31
Fexofenadine HCl	153439-40-8	Inhouse
Bosentan	147536-97-8	Inhouse
D-Cysteine	921-01-7	Inhouse
D-Phenylalanine	673-06-3	Inhouse
Linagliptin	668270-12-0	Inhouse
Rivaroxaban	366789-02-8	USP
Saxagliptin	361442-04-8	USP
Vildagliptin	274901-16-5	USP

Major Pharmaceutical Intermediates

Items Descripation	Structure	Application
MICA ESTER CAS No: 246035-38-1 Purity: ≥98%		For Cefixime
EHATA CAS No: 64485-82-1 Purity: ≥98%		For Ceftazidine
2-Chloroadenine CAS No: 1839-18-5		For Cladribine, Fludarabine et al
Bicyclo(2,2,1)Heptane-2,3-di-exo-carboximide CAS No: 14805o-29-9		For Lurasidne
(R,R)-1,2-Bis(methanesulfonyloxy methyl)Cyclohexane CAS No: 186204-35-3		For Lurasidone
3-(Piperazin-1-yl)benzol[d] isothiazole CAS No: 87691-87-0		For Lurasidone
Trityl olmesartan CAS No: 144690-92-6 Purity: ≥98%		For olmesartan
3-Acetyl Pyridine CAS No: 350-03-8		For Risedronate Sodium
3-(AceticAcid)pyridine HCL CAS No: 6419-36-9		For Risedronate Sodium
Risedronic Acid CAS No: 105462-24-6		For Risedronate Sodium
3-Hydroxy-1-adamantyl-D-Glycine CAS No: 709031-29-8		For Saxagliptin
(1s,3s,5s)-3-(aminocarbonyl)-2-azabicyclo(3,1,0) hexane-2-carboxylic acid tert-butyl ester CAS No: 361440-67-7		For Saxagliptin
(S)-N-Boc-3- hydroxy-adamantylglycine CAS No: 361442-00-4		For Saxagliptin
2-Azabicyclo[3.1.0] hexane-3-carbonitrile, (1s,3s,5s)- CAS No: 866083-42-3		For Saxagliptin
Ethyl 3-(pyridin-2-ylamino) propanoate CAS No: 103041-38-9		For Dabigatran
N-(4-Cyanophenyl) glycine CAS No: 42288-26-6		For Dabigatran
4-methylamino-3-nitrobenzoic Acid CAS No: 41263-74-5		For Dabigatran
S-3-Amino-3-phenylpropanoic acid ethyl ester HCL CAS No: 167834-24-4		For Dapoxetine
(S)-3-Amino-3-Phemylpropan -1-ol CAS No: 82769-76-4		For Dapoxetine
(S)-3-Dimethylamino-3-Phemylpropanol CAS No: 82769-75-3		For Dapoxetine
4-{4-[4-(hydroxydiphenylmethyl)-1-piperidinyl]-1-butynil}-α,α-dimethyl benzene acetic acid CAS No: 832088-68-3		For Fexofenadine HCl
Methyl 2-(4-(4-chlorobutanoyl)phenyl)-2-methylpropanoate CAS No:154477-54-0		For Fexofenadine HCl
5-Bromo-2-chlorophenyl)(4-ethoxyphenyl)methanone CAS No 461432-22-4		For Dapagliflozin
4-(5-Bromo-2-chlorobenzyl)phenyl ethyl ether CAS No :461432-23-5		For Dapagliflozin

Mica Ester,Pharma Intermediates,Ciprofloxacin Hcl Uses,Active Pharmaceutical Ingredients

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