Columns are essentially a laboratory consumable for every laboratory. There are many brand specifications for each column, and each column should be analyzed for the relative sample. So, do you often use the column, do you know how to maintain and maintain the column? Let the column last longer? Here are some basic knowledge about the column. First, the use of the column Reversed phase columns are typically stored in methanol or acetonitrile. Be sure to note that the mobile phase used and methanol or acetonitrile are miscible before use. Columns may dry out during storage due to solvent evaporation, so use 10-20 column volumes of methanol or acetonitrile to equilibrate the column before using mobile phase analysis. If the mobile phase used contains a buffer salt, care should be taken to use water as a transition, rather than directly mixing the organic solvent with the buffer salt. Also pay attention to this when rinsing the column after the test. It is also important to note that it is best not to rinse the column with pure water. For non-polar columns, such as C8 and C18, the purity of the column is reduced because pure water cannot wet the surface of the filler and knock down the carbon chain. In addition, when the sample is analyzed, pure water cannot wet the surface of the filler to form a water film, and the sample does not remain, so that the resolution is lowered and the repeatability is poor. Therefore, for a general C8 and C18 column, the ratio of the organic solvent should not be less than 5%. 1. Try to protect the column with a pre-column or guard column; 2. The pH range of most columns is 2-8, try not to exceed the range; 3. Avoid drastic changes in the composition or proportion of the mobile phase; 4. The mobile phase must be filtered and degassed before use; 5. If a polar or particulate buffer is used as the mobile phase, the column should be rinsed clean after the experiment is completed. Rabies is a fatal viral encephalitis which can be effectively prevented by prophylactic measures. The currently available cell culture vaccines used for rabies prophylaxis are used by the standard intramuscular route of administration Im Rabies Vaccine,Delayed Rabies Vaccine,Rabies Vaccine For Humans,Im Rabies Vaccine For Humans Changchun Zhuoyi Biological Co., Ltd , https://www.zhuoyi-bio.com
The regeneration process and solvent rinsing sequence can be found in the following table: 