Release date: 2015-12-03 Cell's Trends Limited Editions re-sorts reviews from Trends magazines in different topics, and each issue of Limited Editions covers the most influential reviews in a field. The theme of this edition of Limited Editions is "A Special Collection of Reviews in Technological Advances", which contains 12 reviews covering advanced technologies in a variety of scientific fields. It is worth noting that these 12 reviews are fully readable. Source: Bio-Exploration Rape Seeds,Oil Seed Rape,Colza Seeds,Rapeseed Seeds XIKE AGRICULTURAL GROUP CO . .LTD. , https://www.laoseed.com
1. TDP-43: gumming up neurons throughprotein–protein andprotein–RNA interactions
Since the discovery of 43 kDa TARDNA binding protein (TDP-43) has been associated with neurodegeneration, studies related to this protein have focused on the effects of TDP-43 expression regulation on cellular metabolism and survival. This review focuses on two biochemical properties of TDP-43: the ability to bind RNA and protein-protein interactions; and specifically summarizes how these two properties affect important pathological processes.
2.MultiBac: expanding the researchtoolbox for multiprotein complexes
Protein complexes are made up of many subunits that perform most of the most basic processes in cells; therefore, they have become the focus of research. However, producing protein complexes of sufficient quality and quantity is a challenging task in deciphering their structure and function. To overcome this bottleneck, scientists are developing powerful recombinant expression technologies. This review describes one of these technologies, MultiBac, a baculovirus expression vector system.
3.A Crystal Clear Solution For Determining G-protein-coupled Receptor Structures
G-protein-coupled receptors (GPCRs) are medically important membrane proteins that target more than 30% of small molecule drugs. At the time of writing this review, 15 unique GPCR structures have been identified; this opens up new opportunities for drug development and understanding of the molecular mechanisms of GPCR activation. There are many different factors that contribute to the current success, but if one of the factors is to be presented separately as the basis for generating a well-diffracting GPCR crystal, it would be "the stabilisation of the detergent-solubilised receptor-ligand complex". This review will focus on one of the successful strategies for stabilization of GPCRs, namely thermostabilisation of GPCRs.
4.Structural dynamics of nucleosomes at single-molecule resolution
Revealing the structural dynamics of nucleosomes is a crucial step in elucidating the regulatory mechanisms of genomic accessibility. The advances in single-molecule technology have made such studies unprecedentedly efficient. This review summarizes studies of nucleosome structure and kinetics by single-molecule techniques.
5.Experiences In Fragment-Based Drug Discovery
Fragment-based drug discovery (FBDD) has become an alternative to large-scale screening drugs in industry and academia to identify small compounds (fragments) that bind to drug targets. This review summarizes the success stories of the three recent FBDD areas.
6.Drug-Mediated Toxicity: Illuminating The 'bad' In The Test Tube By Means Of Cellular Assays?
Global health problems are rising due to lifestyle changes, increased ageing, and a surge in bacterial antibiotic resistance. However, over the past decade, we have witnessed tremendous developments in the biological “toolboxâ€, especially in areas such as cell testing, stem cell differentiation, and organ imitation systems. These systems are suitable for the identification of lead compounds, however, their use as toxicology test systems is still lagging behind, especially in the absence of regulation. This review aims to clarify the size of the problems that currently exist and to discuss the applicability of these tests, especially the use of stem cells.
7.Yeast two-hybridmethods and their applications in drug discovery
The yeast two-hybrid (Y2H) method was first reported more than 20 years ago and soon became a major breakthrough in protein interaction research and analysis. Since the formation of the method, Y2H has been continuously developed, and on this basis, a variety of experiments have been developed for drug development. This review summarizes the different transcriptional Y2H methods that can be directly used for drug development.
8.Structure-based drug screening for G-protein-coupled receptors
G-protein-coupled receptors (GPCRs) represent a large family of signaling proteins; GPCRs have undergone significant advances in structural biology over the past four years. This article is a review by Brian K. Kobilka, winner of the 2012 Nobel Prize in Chemistry, focusing on the current research advances in silico docking for GPCRs.
9.Behavioral barcoding in the cloud: embracing dataintensive digitalphenotyping in neuropharmacology
For decades, studying the effects of individualized drugs and genetic mutations has become the core of understanding and treating mental disorders. High-throughput technologies derived from other disciplines are changing the scale of behavioral neuroscience data collection. However, collecting and comparing data from different trials has become a major challenge in this area. This review mainly introduces a program called "behavioral barcoding".
10. Selection, Breeding And Engineering Of Microalgae For Bioenergy And Biofuel Production
Microalgae production technology is increasingly attractive for improving energy security and reducing carbon dioxide emissions. This review focuses on the selection, propagation and improvement of microalgae in order to increase biomass and biofuel conversion efficiency.
11.Process engineering of human pluripotent stem cells for clinical application
Human embryonic stem cells (hPSCs), including those derived from embryonic stem cells and induced pluripotent stem cells, are currently the technology backbone of cell therapy. However, to build large-scale production and storage of embryonic stem cells, it is not easy to establish strict control conditions to produce high-quality cells that meet clinical requirements.
This review explores the main elements of human embryonic stem cell production and describes the effects of environmental factors in this process, new three-dimensional culture techniques, and integrated bioreactor methods. Understanding the progress of hPSC in recent years will help to establish a better production platform and develop new hPSC-based cell therapies.
12. Semiconductor quantum dots for in vitro diagnostics and cellular imaging
The need for companion diagnostics, POCT, and high-throughput screening in the field of clinical diagnostics and personalized medicine has driven the emergence of another need to capture more biological information from a single sample of very low concentration and capacity. Optical biosensors based on semiconductor quantum dots (QDs) can meet this need. This review summarizes the applications of recent QDs in in vitro diagnostics and cell imaging.