Application of polyglutamic acid

Polyglutamic acid (γ-PGA), which is a water-soluble, biodegradable, non-toxic, biopolymer produced by microbial fermentation. γ-PGA polyglutamic acid is a viscous substance that was first discovered in the “natto”, fermented bean. γ-PGA polyglutamic acid is a special anionic natural polymer composed of amide linkage between α-amino group (α-amino) and γ-carboxyl group (γ-caboxyl). The molecular weight of homo-polyamide γ-PGA ranges from 50,000 to 2 million Daltons.

Polyamino acid is a new generation of plant nutrition promoter, which has the functions of reducing fertilizer loss, enriching nutrients, improving fertilizer utilization, promoting crop root development and protein synthesis, thereby increasing yield and improving quality.

Agricultural application

Γ-polyglutamic acid γ-PGA can be used as a plant to increase nutrients

1. γ-polyglutamic acid γ-PGA Hydrogel and γ-PGA super hydrophilicity and water retention capacity

When flooded in the soil, a film is formed on the surface of the root hair of the plant, which not only has the function of protecting the root hair, but also is the best transport platform for the intimate contact between nutrients, water and root hair in the soil, and can effectively increase the fertilizer. Dissolution, storage, transport and absorption. Prevents the precipitation of sulfate, phosphate, oxalate and metal elements, enabling crops to more effectively absorb phosphorus, calcium, magnesium and trace elements in the soil. Promote the development of crop roots and strengthen disease resistance.

2. γ-polyglutamic acid γ-PGA Hydrogel and γ-PGA balance soil pH value

It has excellent buffering capacity for acid and alkali, can effectively balance soil pH value, and avoid acid soil caused by long-term use of chemical fertilizer.

3, γ-polyglutamic acid γ-PGA Hydrogel and γ-PGA can be combined with precipitation of toxic heavy metals

It has excellent chelation effect on toxic heavy metals such as Pb+2, Cu+2, Cd+2, Cr+3, Al+3 and As+4.

4, γ-polyglutamic acid γ-PGA Hydrogel and γ-PGA can enhance plant disease resistance and stress resistance

Integrate plant nutrients and water active ingredients in the soil to enhance the resistance to symptoms caused by soil-borne plant pathogens.

5. Promote increased production

It can increase the yield of agricultural products such as tea, fruits and vegetables, and increase the output by 10-20%.

9 planting applications

1. Application of polyglutamic acid fertilizer synergist in vegetable planting

Experimental time: March-May 2010

Experimental method: The cell test of polyglutamic acid fertilizer synergist was mainly carried out in a greenhouse. At this time, the experimental method is to mix different amounts of crude polyglutamic acid extract (provided by Nanjing University of Technology) with fertilizer, and then apply the fertilizer adsorbed with polyglutamic acid as the base fertilizer of the crop, and the later field management is the same as the Daily management of crops.

Experimental results: Polyglutamic acid has obvious yield and fertilizer-saving effects on various crops, and there is no experiment to increase yield. The yield of polyglutamic acid treatment is also comparable to the control treatment using 100% base fertilizer. The results are shown in Table 1.

Table 1 Growth effect of various crops after using polyglutamic acid

Crop name polyglutamate dosage weight loss percentage compared with the control yield increase

Chinese cabbage 10g- 40%+ 9.6%

Rape 10g- 50%- 0.8%

10g + 9.0%

Tomato 10g- 50%+ 14.5%

30g- 50%+ 25.3%

Carrot 30g-20%- 0.7%

50g- 20%+ 1.1%

Artemisia argyi 50g0+ 7.9%

50g- 50%0

Second, the application of polyglutamic acid fertilizer synergist in rice planting

Experimental time: May-August 2009

Experimental method: The test crop was rice cultivar 131, the transplanting size was 30×10 cm, the number of transplanted plants per hole was 5-7, and the date of transplanting was May 23, 2008. Approximately 75 grams of crude polyglutamic acid is blended in the fertilizer used per acre. The specific fertilization amount and results are shown in Table 2.

EXPERIMENTAL RESULTS: Rice field results showed that treatment with 20% weight loss was comparable to control, 40% of treatments were weak and control, and treatment without weight loss increased polyglutamate was better than control.

Table 2 Polyglutamic acid fertilizer effect test field survey data (rice)

Serial number processing name plant height cm square

Number of squares

Number of square meters

Number of turns

1 Local conventional fertilization (pure nitrogen 7kg/mu, P2O5 3kg/mu, K2O 2.5kg/mu) 6632653466

2 polyglutamic acid blending fertilizer 30kg / mu, as base fertilizer application 65326601501

3 polyglutamic acid blended fertilizer 24kg / acre, as base fertilizer applied 6532262436

4 polyglutamic acid blended fertilizer 18kg / mu, as base fertilizer application 6332587407

Note: The formula of polyglutamic acid blending fertilizer is: N 23.3%, P2O5 10%, K2O 17.3%, 0.25% polyglutamic acid (PGA); treatment 1 conventional fertilization; treatment two conventional fertilization application PGA; treatment three increase Apply PGA and lose weight by 20%; treat four additional PGA and lose weight 40%.

Third, the application of polyglutamic acid fertilizer synergist in corn planting

Experimental time: June-November 2009

Experimental method: The test crop is corn, treatment 1: 10 kg of nitrogen fertilizer per mu; treatment 2: 10 kg + 5 g polyglutamic acid per mu; treatment 3: 20 kg per mu of nitrogen fertilizer; treatment 4: 20 kg per mu of nitrogen fertilizer 5 g polyglutamic acid. Fertilization method: nitrogen fertilizer and polyglutamic acid crude product blended and applied.

The experimental results showed that the plant height of the treated two and the treated four maize plants increased by 3.7% and 5.6%, respectively, and the yield increased as shown in Table 3. At the same time, the budget for the application of polyglutamic acid to corn fields was budgeted. According to the corn experiment conducted in Sunjiazhuang Village, Donggang Town, Qinhuangdao City, Hebei Province in 2008, the addition of polyglutamic acid can reduce the use of chemical fertilizer by more than 10%. The yield of corn increased by more than 6%, the input of mu was 11.25 yuan, and the increase of mu was more than 40 yuan. The results are shown in Table 4.

Table 3 Polyglutamic acid fertilizer effect test field survey data (corn)

Processing method processing one processing two processing three processing four

Production / kg1516.817.520

Plant height / cm161167171170

Increasing yield compared with treatment one /%012%16.7%33.3%

Table 4 Economic calculation of corn planting

Treatment of fertilizer (/%) base fertilizer / kg cost savings (/ yuan) PGA increase cost (/ yuan) combination increase cost (/ yuan) mu increase production (/%) mu increase income (/ yuan)

I102.5-1011.25+1.257.656.4

II205-2011.25-8.756.548.58

Note: 25kg compound fertilizer for corn planting, the price of compound fertilizer is 4,000 yuan/ton; the experimental treatment increases the application of 30g of crude PGA per acre, the yield of conventional fertilization is 530kg, the yield of 10% of weight loss is 570.3kg, and the weight loss is 20%. The yield per mu is 564.7kg. When the economy is estimated, the price of corn is 1.4 yuan/kg, and the price of agricultural grade PGA is 150 yuan/kg.

Application of Polyglutamic Acid Fertilizer Synergist in Flue-cured Tobacco Planting

Experimental time: March-July 2009

Experimental method: In the top dressing stage, the effects on the yield and quality of tobacco leaves were investigated by adding different concentrations of polyglutamic acid to the special fertilizer for tobacco. The test group (1 mu) and the control group (1 mu) were planted and the field management was the same. In the experiment, a group of topdressing period was applied with 0.1% polyglutamic acid, the test group 2 polyglutamate was added in an amount of 0.08%, the test three groups were added with 0.05% polyglutamic acid, and the control group was applied with ordinary fertilizer. .

Experimental results: the application of fertilizers with different concentrations of polyglutamic acid for tobacco leaves, the yield of tobacco leaves, the middle and upper smoke rate and the output value were significantly improved, and the special fertilizer for tobacco leaves with 0.1% polyglutamic acid added was the best, which made The output of flue-cured tobacco increased by 18.57%

Table 5 Effect of different polyglutamic acid additions on flue-cured tobacco yield

Treatment control group test one group test two groups test three groups

Production (kg/mu) 150.5178.45169.85158.1

Increasing yield (%) 18.5712.865.04

Superior smoke rate (%) 35484542.5

Moderate smoke rate (%) 41303234

Production value (yuan/mu) 1000128912001125

Value-added rate (%) 28.92012.5

γ-PGA can increase the absorption of nutrients by crops, increase fertilizer efficiency, increase yield and improve quality. γ-PGA is used as a fertilizer synergist, and the properties of the biodegradable absorption enhancer are fully utilized and utilized. It can further increase the yield of crops, and can also provide a good sustained release effect on fertilizers, so that the release time of fertilizers in the soil is about 4 times longer than that of traditional fertilizers. While promoting the absorption of crops, it also inhibits the rapid decomposition and loss of fertilizer components. When γ-PGA is used, the utilization rate of nitrogen fertilizer can be increased by 60.3% on average, and the utilization rate of phosphate fertilizer can be increased by 5.3% on average. The average can be increased by 16.7%, and the comprehensive direct reduction of fertilizer use is more than 20%. Reducing the environmental pollution caused by excessive or improper application of chemical fertilizers has a significant effect of increasing yield and reducing fertilizer.

The characteristics of the product are summarized as follows:

(1) Good effect: After blending with urea, carbon ammonia, compound fertilizer and special fertilizer, it can greatly reduce its decomposition rate and leaching loss, and extend the fertilizer efficiency period from 30 days to 120 days. 30% increased to 95%.

(2) High efficiency: the use of this agent can eliminate l-2 times of topdressing, prevent soil compaction caused by multiple fertilizer application, and greatly reduce farmers' labor intensity and production cost.

(3) Efficacy: This agent contains a variety of trace elements, which have significant disease resistance, drought resistance and lodging resistance to crops. Since γ-polyglutamic acid has surfactant properties, it can significantly improve the efficacy of pesticides by mixing with pesticides and herbicides. At the same time, a variety of long-acting compound fertilizers and special fertilizers can be produced by using the agent. Whether in terms of social benefits or economic benefits, there will be a huge response, which may trigger a revolution in fertilization.

2. Significant application effect of crops

(1) γ-PGA has significant application effects on crops including rice, wheat, corn, vegetables, fruit trees, flowers and the like. Since γ-PGA is highly soluble in water, it contains more than 1000 super-hydrophilic groups (-COOH), which can fully maintain the moisture in the soil, improve the bulkiness and porosity of the sticky soil, and improve the sandy soil. The ability to maintain fertilizer and water retention. γ-PGA has extremely strong water absorption and moisturizing properties, and the maximum natural water absorption ratio can reach 1108.4 times, which is more than twice as high as the currently marketed polyacrylate type water absorbing resin, and the absorption ratio to soil moisture is 30-80 times. The immersion liquid of γ-PGA has certain water retention capacity and ideal release effect in the soil. It has obvious anti-drought and promote seedling effect in dry areas, and can effectively improve the dissolution, storage, transportation and transportation of fertilizer.

γ-PGA also has multiple electrical properties, which can effectively prevent the combination of SO42-, CO32-, PO43-, C2O42- with Ca, Mg and other trace elements in chemical fertilizers, avoiding the formation of low-solubility salts and precipitation, and making crops more It can effectively absorb trace elements in soil, promote the development of crop roots and strengthen disease resistance. It has excellent buffering capacity for acid and alkali, can effectively balance soil pH value, and avoid acid soil and soil plate formation caused by long-term use of chemical fertilizer. In addition, for heavy metal ions such as Pb2+, Cu2+, Cd2+, Cr3+, Al3+, and As4+, γ-PGA has excellent chelation effect and can be used as a heavy metal absorbent to prevent crops from absorbing too much toxic heavy metals in the soil.

2. Application effect of γ-PGA

Applying γ-PGA fertilizer synergist 10-50g per acre, it can promote the accumulation of main nutrient elements, promote root development and increase disease resistance. The application of γ-PGA can generally increase the efficiency of chemical fertilizer application by 10-30%, increase the yield of crops such as tobacco and vegetables by 8-31%, increase the yield of rice, improve the quality of crops, and achieve a better sustained release effect on fertilizers. The sustained release time of the fertilizer in the soil is extended by about 4 times compared with the traditional fertilizer, which promotes the absorption of the crop and also inhibits the rapid decomposition and loss of the fertilizer component. Especially in the low-fertility soil, the application effect is better, and the yield increase is larger. It can directly reduce the amount of fertilizer by more than 20%, and at the same time, it is degraded by microorganisms in the soil, and the environmental pollution caused by excessive or improper application of fertilizer is greatly reduced. Improve the ecological environment of the countryside.

The field plot experiment of γ-PGA compound fertilizer showed that the yield of pakchoi could be increased by more than 20%, and the yield of lettuce could be increased by more than 15%. The tobacco seedling hydroponic test and pot experiment showed that γ-PGA could increase the yield of tobacco, the hydroponic test increased by more than 15%, and the pot experiment increased by more than 20%.

After 2 times of PGA with 50, 100, 200 mg/L, the yield of strawberry increased by 30.4%, 29.5% and 19.9% ​​compared with the control, which produced a significant yield increase effect. After the strawberry seedlings were planted, the 100mg/L PGA treatment accelerated the growth rate of the roots of the plants. The average root length was significantly different from the control; the leaf thickness was increased, and the leaf thickness was significantly different from the control; The soluble solids increased by 2.6%, 11.4% and 3.9%, respectively. The results of colorimetric test showed that the L value of fruit, the C value of coloring intensity and the red coloring value a increased compared with the control after 100 mg/L PGA treatment. The surface of the fruit is more lustrous.

3. Agricultural γ-PGA product form and application method

At present, the agricultural γ-PGA product forms mainly include γ-PGA nutrient solution and instant γ-PGA full essence dry powder. The method of application is as follows: 20 g/mu γ-PGA (effective substance content), which can be applied alone or in combination with other fertilizers.

Both γ-PGA products use low molecular weight γ-PGA as the main raw material, and γ-PGA nutrient solution has the characteristics of strong water retention, easy absorption, and long-term sustained release. The main functions are as follows:

(1) Enriching nutrients and improving fertilizer utilization rate;

It has the characteristics of anionic surfactant. On each residue of its long chain of protein, there is a free negatively charged carboxyl group, which has strong chelation function for positive electrotrophic segregation. The adsorption exchange capacity with nutrient segregant is natural soil. About 100 times, it reduces the leaching and volatilization of nutrients. While enriching nitrogen, phosphorus and potassium, it also enriches the micro-nutrients such as calcium, magnesium, molybdenum and zinc, which satisfies the various needs of crops and greatly improves the soil. The supply capacity of nutrients. After use, the utilization rate of nitrogen fertilizer is increased by 50~60%, the utilization rate of phosphate fertilizer is increased by more than 5%, the utilization rate of potassium fertilizer is increased by 15~20%, the fertilizer can be saved by 10~20% per mu, and the symptoms of deficiency are not easy to occur.

(2) Long-term slow release function

The adsorption and sequestration functions of nutrients are stored, and the nutrients temporarily used by plants are stored and slowly released as the crop grows and develops, so as to meet the nutrient demand in the middle and late crops, avoid premature aging and de-fertilization, and reduce the number of top dressings. And the amount of topdressing, the basic fertilizer can be applied to the food crops to meet the nutritional needs of the whole growing season. There is no need to topdress, which greatly reduces the labor intensity.

(3) Improve soil physical and chemical properties

The γ-PGA nutrient solution promotes the development of roots and also increases the amount of organic matter recovered by the soil. After use, the soil organic matter content increased by more than 10%; it is conducive to the formation of soil aggregate structure, the physical clay content of the particle size between 0.005~0.001mm increased by 1.73~3.13%; the soil bulk density decreased by 3.73%. The product has strong water absorption, which can increase the water content of 0~20cm soil layer by 1.5~2.8%, and increase the water content of 20~40cm by 1~1.3%, thus improving the drought resistance of crops. The increase of organic matter content and the decrease of capacity greatly improve the water retention and fertilizer retention capacity of the soil, and are beneficial to crop metabolism and soil warming in winter.

(4) Strongly promote root development

PGA nutrient solution contains beneficial phytohormones such as auxin and cytokinin produced by microorganisms, which can effectively promote the development of roots, grow longer roots and more root hairs, increase root surface area and increase crop absorption. The ability of nutrients enhances the ability of crops to resist drought, cold, disease and other stresses, and has a certain ability to resist low temperatures. This feature increases the yield of crops such as tuber and tubers, such as potatoes, sweet potatoes, ginger, garlic, onions, radishes, and peanuts.

(5) Improve crop yield and quality

It is an essential nutrient for crops. The coordinated supply of crop nutrients can promote protein synthesis, early maturity, improve product quality, avoid malnutrition and use of hormones to produce deformed fruit, cracked fruit and poor fruit color. After using this product, the protein content of corn increased by 3~6%, the protein content of wheat increased by 20~30%, the soluble sugar of tomato increased by 0.5~1%, the crude protein increased by about 0.1%, and the sugar content of fruit increased by 10~20%. The level of fruit increased, the sugar content of watermelon increased by 8~10%, the glucose content increased by about 10%, the tobacco first-grade smoke increased by about 10~15%, and the proportion of upper-middle smoke increased by about 6%.

(6) Increase production and harvest

When used in combination with chemical fertilizers, grain crops can be increased by 10-15%, vegetables by 30-60%, eggplant crops by 10-15%, forages by 30%, and oil crops by about 10%.

(7) Environmentally friendly

The γ-PGA nutrient solution itself is a nutrient substance, has no toxic side effects, and has no residual toxicity in the soil.

4. The physical control photos of the γ-PGA photos of agriculture are as follows:

(1) Rice

When γ-PGA is applied to rice, it can increase the seed setting rate and protein content of rice, and the yield increase effect is obvious.

Figure 5 Comparison of rice application γ-PGA products

(2) Leafy crops

Spraying γ-PGA powder on leafy crops can promote the growth of plants, green leaves, effective prevention of deformed fruit, enhanced resistance to stress, and reduction of physiological diseases, which can significantly increase yield of leafy crops by more than 30%.

Figure 6 Comparison of γ-PGA product application in vegetables

Figure 7: Comparison of lettuce application γ-PGA product

(3) Solanum crops

γ-PGA is applied to the solanaceous crops, which can significantly increase yield by more than 10%, so that the fruits are even, the coloration is good, and the taste is improved.

Figure 8: Comparison of tomato application γ-PGA product

(4) Fruit

γ-PGA was applied to the planting of sakura, and γ-PGA was applied to the peach. The fruit was even, the coloration was good, the fruit setting rate was improved, the taste was crisp and sweet, and the yield increased significantly.

Figure 10 Cherry γ-PGA product comparison chart

Strawberry spraying γ-PGA, leaves thick, green color, increased plant height and plant growth, improve crop photosynthesis, effectively enhance disease resistance, strawberry quality improved, yield increased by more than 10%.

Figure 11: Comparison of strawberry application γ-PGA product

(5) Other

When γ-PGA is applied to the turf, the root amount can be increased, the root length is deep, the thick root grows, and the leaves are thicker green.

Figure 12 turf application γ-PGA product comparison chart

5. γ-polyglutamic acid γ-PGA can be used as a plant to increase nutrients

1. γ-polyglutamic acid γ-PGAHydrogel and γ-PGA super hydrophilicity and water retention capacity

When flooded in the soil, a film is formed on the surface of the root hair of the plant, which not only has the function of protecting the root hair, but also is the best transport platform for the intimate contact between nutrients, water and root hair in the soil, and can effectively increase the fertilizer. Dissolution, storage, transport and absorption. Prevents the precipitation of sulfate, phosphate, oxalate and metal elements, enabling crops to more effectively absorb phosphorus, calcium, magnesium and trace elements in the soil. Promote the development of crop roots and strengthen disease resistance.

2. γ-polyglutamic acid γ-PGAHydrogel and γ-PGA balance soil pH value

It has excellent buffering capacity for acid and alkali, can effectively balance soil pH value, and avoid acid soil caused by long-term use of chemical fertilizer.

3, γ-polyglutamic acid γ-PGAHydrogel and γ-PGA can be combined with precipitation of toxic heavy metals

It has excellent chelation effect on toxic heavy metals such as Pb+2, Cu+2, Cd+2, Cr+3, Al+3 and As+4.

4, γ-polyglutamic acid γ-PGAHydrogel and γ-PGA can enhance plant disease resistance and resistance to stress

Integrate plant nutrients and water active ingredients in the soil to enhance the resistance to symptoms caused by soil-borne plant pathogens.

5. Promote increased production

It can increase the yield of agricultural products such as tea, fruits and vegetables, and increase the output by 10-20%.

Ingredients: 1. Total nitrogen 1.5% 2, total phosphoric anhydride 0.1% 3, total potassium oxide 0.1%

Usage: Dilute 300 times directly around the root (filling head) or flood irrigation field, apply once every 15 to 20 days (can be combined with 1000 times dilution to apply foliar spray).

Usage: Rice, vegetables, melons, turf, flowers, tea gardens, etc. 0.15-0.5 liters/mu.

Application time: Plants and crops can be applied in appropriate amounts in different growth stages. It is not recommended for 15 days before tea picking.

Precautions:

1, with the base fertilizer, top dressing, chemical fertilizer application at the same time, the effect is very obvious, the best time is the second day after the flat fertilization, the third day of the mountain.

2, can be used at the same time as pesticides, but pesticides should not exceed the recommended measurement.

Website: Polyglutamic acid (γ-PGA) in agricultural production

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