Photometric analysis is one of the most commonly used quantitative analysis methods. In photometric analysis, the selection, use and maintenance of cuvettes have an important impact on the analytical results. Leber came to talk about the choice of cuvette in the photometric method.

For analysis wavelengths above 350 nm, we can use glass or quartz cuvettes, and quartz cuvettes must be used below 350 rll/i.

Cuvettes have different optical path lengths, generally 0.5, 1, 2, 3, and 5 ∞. The choice of which optical path length cuvette should be based on the absorbance of the sample. When the color of the colorimetric liquid is lighter, a 2 cm or 3 ∞ cuvette with a longer optical path length should be used; when the color of the colorimetric liquid is darker, a lower optical path length such as 0.5 should be used. m, lIII cuvettes so that the absorbance of the measured solution is between 0.1 0.7.

The cuvette is directional. Some cuvettes are marked with a directional mark and must be used when using. Cuvettes without direction marks should be calibrated, and the direction must be determined and marked before calibration. To reduce the measurement error. The cuvette is calibrated by injecting pure distilled water into the cuvette, setting the absorbance of the cuvette with the least absorption to zero, and using this as a reference to measure the relative absorbance of the other cuvettes. When measuring a colorimetric solution, the absorbance of the cuvette should be subtracted from its absorbance. The difference between the same set of cuvettes should be less than the measurement error. When measuring the same solution, the difference in absorbance should be less than 0.5%, otherwise the difference should be corrected.

The optical path length of the cuvette is also corrected. When the calibration is performed, a solution having an absorbance of about 0.4 can be separately injected into the calibration dish and a standard cuvette having an accurate path length to measure the absorbance. The absorbance in the standard cuvette is 1. O0, the relative value of the corrected cuvette absorbance is obtained as the correction coefficient of the cuvette. When the colorimetric solution is measured, the measured absorbance can be multiplied by the correction factor of the dish to obtain the actual absorbance value.

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